Armatimonas rosea is a Gram-negative bacterium and also the first species to be characterized within the phylum Armatimonadetes. The Armatimonadetes were previously known as candidate phylum OP10. OP10 was composed solely of environmental 16S rRNA gene clone sequences prior to A.rosea's discovery.
Discovery
A. rosea was originally isolated from the rhizoplane of the aquatic plant Phragmites australis inhabiting a freshwater lake in the Yamanashi prefecture in Japan. Cultivation and isolation were completed in a low nutrient medium, DTS.
Relatives
The environmental 16S rRNA gene sequences, belonging to the phylum Armatimonadetes are currently sorted into six groups. Groups 2, 5, and 6 consist solely of sequences. Group 1 contains Armatimonadetes rosea, Group 3 contains Chthonomonadetes calidirosea, and Group 4 conatins Fimbriimonas ginsengisoli.
Chthonomonas calidirosea strain T49T, an aerobic, saccharolytic, obligately thermophilic, motile, non-spore-forming bacterium, was isolated from geothermally heated soil at Hell’s Gate, Tikitere, New Zealand. It is the first representative of a new class in the phylum Armatimonadetes. It represents the first cultured representative of the Chthonomonadetes, corresponding with Group 3 of the phylum Armatimonadetes.
Fimbriimonas ginsengisoli, an aerobic, non-motile, mesophilic, rod-shaped bacterium, was isolated from a ginseng field soil sample. It represents the first cultured representative of the Fimbriimonadia class, corresponding with Group 4 of the phylumArmatimonadetes.
Characteristics
When grown on the relatively low-nutrient medium, R2A, colonies were pink pigmented, circular, smooth, significantly hard, relatively small (1â€"2 mm) in size after one week of incubation at 30oC. A.rosea has an optimum temperature at 30-35oC with a range of 20-40oC. The optimum pH for growth was 6.5 with a range of 5.5-8.5. It is able to tolerate NaCl concentrations up to 0.5%.
When examined by phase-contrast and transmission electron microscopy cells were observed to be nonmotile, ovoid to rod shaped, with sizes ranging from 1.4-1.8 µm in width and 2.4-3.2 µm in length. Neither spores nor flagella were observed.
When investigated in R2A media, cultures of A. rosea were observed to be aerobic and chemoheterotrophic with no evidence of growth under anaerobic conditions. It is negative for the following tests: nitrate respiration, fermentative growth, catalase, and cytochrome oxidase.
References
1. Tamaki, Hideyuki, et al. "Armatimonas rosea gen. nov., sp. nov., of a novel bacterial phylum, Armatimonadetes phyl. nov., formally called the candidate phylum OP10." International journal of systematic and evolutionary microbiology 61.6 (2011): 1442-1447.http://ijs.sgmjournals.org/content/61/6/1442.short
2. Lee, Kevin C-Y., et al. "Chthonomonas calidirosea gen. nov., sp. nov., an aerobic, pigmented, thermophilic micro-organism of a novel bacterial class, Chthonomonadetes classis nov., of the newly described phylum Armatimonadetes originally designated candidate division OP10." International journal of systematic and evolutionary microbiology 61.10 (2011): 2482-2490.http://ijsb.sgmjournals.org/content/61/10/2482.short
3. Im, Wan-Taek, et al. "Description of Fimbriimonas ginsengisoli gen. nov., sp. nov. within the Fimbriimonadia class nov., of the phylum Armatimonadetes."Antonie van Leeuwenhoek (2012): 1-11. http://www.springerlink.com/content/m213r21t1g2603q3/
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